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Uhlén Lab - Downloads

Protocols

Protocols for calcium imaging buffers
» pdf

Immuno staining of cultured cells
» pdf

Immuno staining of tissue slices
» pdf

Software

Spectral Analysis

SpectralAnalysis (Version 3.0) is a tool written for MATLAB that performs spectral analysis on calcium oscillation data. The zipped file (SpectralAnalysis-v3.zip) includes the main file and program (SpectralAnalysis.m), the subroutines that read files into the program (readFile.m), the subroutines that write information to files (writeFile.m), instructions on installing and executing the program (readme.txt), and three different samples of oscillation data (samples.txt). A file called standard.ini, which does not come with the zipped files, will be created by the SpectralAnalysis program the first time it is executed and stores the latest input values entered by the user.
[Download SpectralAnalysis-v3.zip]

Pick Cells

PickCells (Version 1.0) is a tool written for MATLAB that helps you to select out different calcium responding cells from a set of recordings.
[Download PickCells.zip]

Publications

Selected publications available for download.

Uhlén P, Fritz N, Smedler E, Malmersjö S, Kanatani S "Calcium Signaling in Neocortical Development"
Dev Neurobiol Apr;75(4):360-8 (2015) Review
» pdf

Smedler E, Malmersjö S, Uhlén P "Network Analysis of Time-Lapse Microscopy Recordings"
Front Neural Circuits. Sep 17;8:111 (2014) Method
» pdf

Smedler E, Uhlén P "Frequency Decoding of Calcium Oscillations"
Biochim Biophys Acta. Mar;1840(3):964-9 (2014) Review
» pdf

Uhlén P, Fritz N "Biochemistry of Calcium Oscillations"
Biochem Biophys Res Commun. May 21;396(1):28-32 (2010) Review
» pdf

Uhlén P "Spectral Analysis of Calcium Oscillations"
Science STKE Nov 09;(258):pl15 (2004) Method
» pdf

Bäck T, et al. "An educational tool For Demonstrating the TOF-PET Technique"
Nuclear Instruments and Methods in Physics Research A 471 200-204 (2001)
» pdf

Useful reading

"The Clean Microscope" from Zeiss
» pdf

"Microscopy from the very beginning" from Zeiss
» pdf

"Basics of Light Microscopy & Imaging" from Olympus
» pdf

 

[ Department of Medical Biochemistry and Biophysics | Karolinska Institutet ]
Updated: March 4, 2019